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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 13 No 2 (2008): June 2008" : 15 Documents clear
PEMANFAATA N EKSTRAK DAUN WUNGU {Graptophyllum pictum (L.) Griff.} UNTUK PENURUNAN KADAR KOLESTEROL SERUM DARAH MENCIT BETINA YANG DIOVARIEKTOMI Listijani Suhargo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/354

Abstract

The purpose of this research was conducted to evaluate the effects of “daun wungu” extracts to decrease total cholesterol, LDL (Low Density Lipoprotein) and HDL (High Density Lipoprotein) cholesterol of ovariectomized mice. This research used 24 ovariectomized mice and 8 normal mice. The ovariectomized mice were grouped in 3 groups for K1 (the treatment with aquadest, 0.05 ml), K2 (the treatment with fish oil, 0.05 ml) and P (the treatment with “daun wungu” extracts, 0.5 mg in 0.05 ml fish oil). All treatments were done for 20 days. At the end of the treatments, the blood (0.5 ml) was taken from cardiac by tuberculin disposable syringe. And then with fotometry, serum total, LDL and HDL cholesterol were measured. The result of this research showed that daun wungu extracts (0.5 mg in 0.05 ml fish oil) could not decrease HDL cholesterol, but it could decrease total and LDL cholesterol of blood serum in ovariectomized mice.
KAPANG ENDOFIT DARI TAMAN NASIONAL GUNUNG HALIMUN SEBAGAI PENGHAMBAT PERTUMBUHAN MIKROBA PATOGEN Salmonella thypi DAN Candida albicans Ruth Melliawati; Puspita Suci Wulandari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/355

Abstract

Endophytic fungi live inside plants without harming the host. The purpose of this research was to screen endophytic fungi which could inhibit Salmonella thypi and Candida albicans, as well as characterizing of endophytic fungi, and antimicrobial compound produced by endophytic fungi. Some methods were used on this research. Diffusion agar plate methode was used to screen endophytic fungi which could produce antimicrobial compound against Salmonella thypi and Candida albicans. Standard plate count was used to measure fungi growth. Antimicrobial compound produced by endophytic fungi was analized by TLC and HPLC technique, compared with standard antibiotic, chloramphenicol and nystatin. The result showed 5 kinds of endophytic fungi produced antimicrobial compounds against Salmonella thypi. The largest zone of inhibition was 115 mm2 shown by Hl.25F.112. Two kinds of endophytic fungi were able to inhibit Candida albicans with the largest inhibiting zone was 164 mm2 shown by Hl.108F.481. The morphology of Hl.108F.481 indicated that this fungus had vertical hypha with sporangium at the end of the hypha and Hl.25F.112 had partioned hypha and oval-shape ascus. TLC and HPLC analysis showed that water extract of Hl.25F.112 (1s), chloroform extract of Hl.25F.112 (1c) and chloroform extract of Hl.108F.481 (2c) contained anti-microbial compound with retention time (RT) closed to chloramphenicol and water phase of Hl.25F.112 (1s) closed to nystatin.
ISOLASI DAN SELEKSI MIKROBA AMILOLITIK DARI MAKANAN FERMENTASI/RAGI TAPAI GAMBUT DI KALIMANTAN SELATAN Elidar Naiola
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/356

Abstract

Fourteen isolates of microbe which produce amylase were isolated from various fermented foods/ragi tapai at South Kalimantan. Three out of them were produce the higher amylase activities compared to others which shown the clear zone areas after pouring with Iodium solution with relative activities > 3. From the studies on morphological and physiological characterization, it was indicated that one isolate was belong to yeast, it was namely Candida sp (ISO RTG). The amylase activity of Candida sp (ISO RTG) was studied in media containing soluble starch, and result showed that the maximum activity of amylase reach after 3 days, it was 1.85 × 102 U/ml (one unit activity is define as μmol of glucose produce per ml per minute). In medium content rice flour as a carbohydrate sources, the maximum activity was 1.91 × 102 U/ml. The optimum temperature for enzyme reaction was at 40–45° C while optimum pH was at pH 5.0–6.0 and the enzyme was relatively stable under such conditions.
DEKLOROFILASI EKSTRAK PROTEASE DARI TANAMAN BIDURI (Calotropis gigantea) DENGAN ABSORBAN CELITE Yuli Witono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/357

Abstract

Biduri’ plant is a wild bush in tropical countries which is one of potential protease source. However, protease extracted from leaf and stamp top of biduri is still green in color due to contain a protein bounding-chlorophyll. It would be problem if it is used for some food. The objective of this rearch was to study a dechloroplyllation technique in order to obtain protease with low chlorophyll content but high specific activity. The results showed that the best dechlorophyllation method of biduri protease could be eluted by celite absorbance. The first step elution was obtained 16 ml filtrate of biduri protease with low chlorophyll. Consist to the result above also decreased protein content, with loading capacity was 1.067 gram biduri/gram celite or 0.015 μg chlorophyll/gram celite. However in the second step elution, after biduri filtrate has been freezed for 12 hours was obtained the dechlorophylated biduri protease was higher in loading volume. Resulting in increased of loading capacity to be 2.13 gram biduri/gram celite or 0.004 μg chlorophyll/gram celite. The chlorophyll decreased to about < 44% of chlorophyll from the first step elution, even the specific activity increased 286% compared with the first step elution.
CUTICULAR COMPONENT ANALYSIS FOR DISCRIMINATION OF Aedes Aegypti (DIPTERA: CULICIDAE) FROM SEVEN LOCALITIES IN SOUTH KALIMANTAN Abdul Gafur
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/358

Abstract

Cuticular components of female Aedes aegypti from seven localities (populations) in South Kalimantan were compared. Mosquitoes from four populations of Banjarmasin, and one population each of Pelaihari, Barabai, and Kotabaru were sampled. Cuticular hydrocarbons were removed from adult female and were separated and quantified by gas chromatography. Stepwise discriminant analysis determined the degree of differences between populations. Using the concentration of the hydrocarbons in a linear discriminant function and the estimator obtained from cross validation, it was demostrated that specimens could be correctly allocated the population to which they belong with an average success rate of 80.6%. All (100%) specimens of populations outside Banjarmasin were correctly identified. There was reduced segregation of the four Banjarmasin populations, suggesting greater similarity in the hydrocarbons of Aedes aegypti from these populations. It was suggested that the greater similarity correlated with increased contact between populations in the region.
PERAN ANTIBODI KUNING TELUR (IgY) SEBAGAI OPSONIN UNTUK PENCEGAHAN SERANGAN MUTAN STREPTOCOCCUS SEROTIPE D (STREPTOCOCCUS SOBRINUS) Okti Nadia Poetri; Retno D. Soejoedono; Agustin Indrawati; I Wayan T. Wibawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/359

Abstract

The aim of this study was to explore the role of serotype d Mutan Streptococcus (Streptococcus sobrinus) spesific immunoglobulin Y (IgY-Ss) as opsonin against the same strain. The eggs were collected from Single Comb Brown Leghorn which has been immunized with Streptococcus sobrinus. Agar gel precipitation test was applied to detect IgY-Ss in serum and egg. Egg containing IgY-Ss was collected and extracted by PEG-Amonium sulphate and purified using fast protein liquid chromatography. The purity of IgY-Ss was determined by UV spectrophotometer. Molecular weight was established by SDS-PAGE (sodium dedocyl sulphate-poly acrilamide gel electrophoresis). Biological activities of IgY-Ss as opsonin was determined by phagocytosis assay. Phagositic activity of macrophages was not increased by preincubation of both S. sobrinus (107 CFU/ml) and 100 μg of IgY-S, however the phagositic capacity was increased from 1.6 bacterial cell/ macrophag to 5.17 bacterial cell/ macrophag. These finding suggest that IgY-Ss obtained from hens immunized with S. sobrinus provide an alternative to prevent S. sobrinus infection.
HUBUNGAN KEKERABATAN BURUNG GELATIK JAWA (Padda oryzivora) DI PULAU JAWA BERDASARKAN KARAKTER MORFOLOGI R. Susanti; Margareta R.; Nugroho E.K.; Lidia Martanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/360

Abstract

The study was conducted to evaluated the phylogenetics of the morphology character of gelatik jawa (Padda oryzivora). Fifty four P. oryzivora from natural population in Surabaya, Malang, Ngawi, Bekasi, Karawang, Cirebon, Bogor, Semarang, Magelang, Yogyakarta and Prambanan is adapted to condition of the research for 2 week. After individual identification card with bird ring and measured of the quantitative and qualitative of morphology characters, morphology characters of the bird were analysed of the phylogenetics. The phylogenetics of P. oryzivora population suggested that the bird from the geographic regions not a grouping. The high degree of migration is due to stress environment, especially of the broken of the forest, hunting, destroying and pesticides application. The migration of the population is due to the traffic of the trade from the region to the other region. This problem press to put the constitution into effect the trade embargo this species. The degree of the individual variation due to the degree of the migration, random breeding and selection. The degree of the inbreeding of the birds is not reported, so need to follow-up studies.
UJI DEGRADASI SELULOSA DARI JAMUR TANAH HUTAN BEKAS TERBAKAR WANARISET-SEMBOJA, KALIMANTAN TIMUR Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/361

Abstract

In order to know the effect of isolation method on the occurrence and capability of soil fungi to degrade cellulose, a study was conducted in an over-burned forest in Wanariset-Semboja, East Kalimantan. Soil fungi were isolated using three isolation methods: incubation at 45° C, treatment with 50% ethanol for 15 minutes, and heat treatment at 70° C for 15 minutes. Plates for heat incubation and for other methods were incubated at 45° C and 27° C for three days, respectively. Cellulose degradation test of isolated fungi was examined using Carboxy Methyl Cellulose (CMC) media. Results showed that isolation method affected diversity and population of soil fungi. Heat treatment at 70° C for 15 minutes appeared to have highest diversity and population of soil fungi. Eupenicillium javanicum var javanicum (van Veyma) Stolk & Scott, Talaromyces byssoclamydoides Stolk & Samson, T. flavus (Klocker) Stolk & Samson, T. stipitatus C.R. Benjamin, and Penicillium argillaceum Stolk et al. were dominant in an over-burned forest in Wanariset-Semboja, East Kalimantan. Twenty-one isolated fungi degraded cellulose.
PERFORMANSI SAPI BALI PADA TIGA DAERAH DI PROVINSI BALI A. Supriyantono; Luqman Hakim; Suyadi; Ismudiono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/362

Abstract

The aim of the research was to know qualitative and quantitative traits of Bali cattle at three different areas geographically (lowland, midland and highland) at Bali province whether there are deviations or not. Variation in coat colour and some description about phenotypic Bali cattle are the main aspect for qualitative trait whereas body weight, some statistical vital (height at hip, body lenght, heart girth) and reproduction traits (mating system, origin of sire, condition of physiology, service per conception, calving interval and days open) of Bali cattle are the main aspect for quantitative trait. Sample of Bali cattle was taken in Tabanan (midland), Pulukan (lowland) and Karang Asem (highland) area about 2% of the total population. Each trait was measured at different age group. The age for each cattle was based on permanent incicy (PI) wich divided into 5 PI (PI0, PI1, PI2, PI3, PI4). The qualitative traits were found by direct observation at the field together with measurement of quantitative traits, body weight and some statistical vital. The result showed that in general Bali cattle are sexually dimorphic spesies, with bull being dark chestnut brown and cow and juveniles reddish brown. Both sexes have white rump patches and leggings. Both sexes carry horns, although they are much heavier and larger in the males.Quantitatively, bali cattle in Pulukan are much smaller than any other area followed in lack reproduction traits.
STUDI PEMANFAATAN KULIT KERANG SEBAGAI BAHAN PENYUSUN PADA PEMBUATAN LEM KACA M. Nadjib M.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/363

Abstract

Shellfish are ones of sea’s product which use as food, and shell part as a waste. This experiment was done to utilize shell as main product in glass adhesive together whit Arabic gum and white part of chicken egg as sticky substances. The shell contended about 76,64% of CaCO3 which could repair adhesive working to increase shear strength and curing time. Adhesive were made by variation composition in gram number of shell powder and Arabic gum. The best composition was obtain on 68.45% of shell; 8.22% of Arabic gum; 1.42% of white part of chicken egg and 21.90% of water. This composition had 16,620.105 NM-2 of shear strength; 23.14% of volatility and 90.9583 pa.det or 909.5830 poise viscosity as optimal result at eleven days of curing time. In environment resistant test, shear strength decreased about 64.33% in acid; 17.96% in water; and 1.83% in base. Storage life test of adhesive was performed in closed box at temperature 27° C and fungi grew it in fourth week.

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